THE LS-ANTIGEN OF VACCINIA

The Ls-antigen of Vaccinia

Studies on LS-protein, the soluble double antigen of vaccinia, and on the degradation products L'S and L''S' have been made with electrophoresis and in the analytical ultracentrifuge. LS, which is homogeneous electrically and in the ultracentrifuge, has an isoelectric point at pH 4.8. At 4 degrees C. its partial specific volume is 0.72 cc./gm., and its diffusion constant is 1.50 x 10(-7) cm.(2)...

THE LS-ANTIGEN OF VACCINIA IV. CHEMICAL ANALYSIS O~" LS AND TIlE EFFECT OF CHYMOTRYPSIN ON LS* BY

Recent observations in our laboratories (1, 2) have demonstrated that a single molecular substance carries all of the serological activity of both the Land Ssoluble antigens of vaccinia. Craigie and Wishart, (3) who originally described these two antigens thought that they occurred in the form of a complex in nature, but, from their own observations (3) and from those of Parker (4), they were l...

Studies on the Soluble Precipitable Substances of Vaccinia Iii. T~e Precipitin Responses of Rabbits to the Ls Antigen of Vaccinia

Skin reactions to vaccinia virus infection in the rabbits immunized with vaccinia-soluble early antigen.

Rabbits were immunized with vaccinia-soluble early antigen (Es antigen) in Freund's complete adjuvant (FCA). Circulating neutralizing antibody was not induced, but CF antibody against the soluble antigens of vaccinia-infected cells was induced. By intradermal inoculation of vaccinia virus, erythema developed early at the site of inoculation and enlarged up to 3 days post infection (p.i.). No mu...

the effect of taftan pozzolan on the compressive strength of concrete in the environmental conditions of oman sea (chabahar port)

cement is an essential ingredient in the concrete buildings. for production of cement considerable amount of fossil fuel and electrical energy is consumed. on the other hand for generating one tone of portland cement, nearly one ton of carbon dioxide is released. it shows that 7 percent of the total released carbon dioxide in the world relates to the cement industry. considering ecological issu...